Importantly, RASD2 has been shown to interact with β-arrestin2, which contributes to the regulation of the DRD2-dependent CREB response element-binding protein pathway in the dopamine system. Mechanistic studies indicated that miR-4763-3p inhibits RASD2 by base-pairing with the 3' untranslated region of RASD2 mRNA. Moreover, positron emission tomography data collected for 44 human samples identified the prefrontal and temporal lobes as potential key brain regions in schizophrenia patients. Indeed, analysis of blood samples confirmed that RASD2 is downregulated in schizophrenia patients. In particular, competing endogenous RNA network revealed the important role of the gene RASD2, which is regulated by miR-4763-3p. To address this serious shortcoming, we used a bioinformatics approach to identify 61 genes that are differentially expressed in schizophrenia patients compared with healthy controls. Indeed, no molecular-level test exists to assist diagnosis, which has limited treatment strategies. These screened siRNAs should effectively silence the RdRp and 3C-like proteinase genes of SARS-CoV-2 by siRNA mediated prospective treatment of COVID-19 patients.Įxisting diagnostic methods are limited to observing appearance and demeanor, even though genetic factors play important roles in the pathology of schizophrenia. Finally, after analyzing the GC ratio (%), free energy of folding-binding, melting temperature and efficacy prediction of all the predicted siRNAs, 6 siRNA molecules were selected which are proposed to exert the highest action. A total of 38 siRNAs were predicted that can inactivate RdRp and 3C-like proteinase genes. 12 conserved sequences were obtained from the genome 101 SARS-CoV-2 strains sequenced in different countries. In the current study, siRNA molecules were designed against RdRp and 3C-like proteinase gene. Among various treatment options, siRNA, also known as small interfering RNA is a prospective tool of the RNA interference (RNAi) pathway that controls virus-mediated infections of human by suppressing the expression of viral gene through hybridization and neutralization of complementary mRNA. ![]() Two proteins, namely, RNA-dependent RNA polymerase (RdRp) and 3C-like proteinase work in facilitating the entry of the virus into host cell, viral transcription and replication. ![]() This has led to the race of scientists all over the world to discover a therapeutic to combat the SARS-CoV-2. Since the origin of the SARS-CoV2 from Wuhan, in China, it has become a global threat to public health and a WHO-declared world epidemic within 2 month of identification of the first case.
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